Specificity of Creatine Phosphokinase

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Cardiac Specific Creatine Phosphokinase

The specificity and sensitivity of serum creatine phosphokinase cardiac specific isoenzyme (MB) in the diagnosis of acute myocardial infarction (AM I) was evaluated. An ion-exchange chromatographic technique was used to isolate MB. Sera layered on mini-columns of DEAE-Sephadex were eluted with Tris-buffered sodium chloride. Quantification of isolated MB was performed by creatine phosphokinase (...

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Increasing interest in serum enzyme levels in various conditions has led to recognition of the value of the estimation of creatine phosphokinase activity in muscular disorders. Interpretation of the results obtained has been hampered by the lack of adequate information about the ;normal range'. Data for the normal levels of creatine phosphokinase in cord blood, children, and adults are presented.

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Plasma creatine phosphokinase (GPK) was estimated from unmedicated schizophrenics to evaluate its significance in the diagnosis. The mean value of CPK in schizophrenics is 78.41±5.78 IU/L (n=49) as against control value of 27.45±2.27 IU/L (n = 36). The statistical analysis showed significance (p<0.05). The study finds its importance in view of the earlier reports suggesting the diagnostic value...

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Creatine phosphokinase in mammalian brain.

Hawk, P. B., Oser, B. L. & Summerson, W. H. (1947). Practical Phy8iotogical Chemi8try. London: Churchill. Heilbrunn, L. V. (1947). An Outline of General Phy8iology. Philadelphia: Saunders. Hoagland, H. & Stone, D. (1948). Amer. J. Phy8iol. 152, 423. Klein, J. R. & Olsen, N. S. (1947). J. biol. Chem. 167, 747. Krebs, H. A. (1950). Biochim. biophy8. Acta, Am8t., 4, 249. Krebs, H. A. & Eggleston, ...

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Determination of serum creatine phos~hokinase (CPK; EC 2.7.3.2.) is important ill laboratory diagnosis of myocardial infarction (M') and skeletal muscle disorders (7). Two recent reports suggest that administration of corticosteroids decreases serum CPK activity (1.5). If patients with MI and myopathies are given. corticosteroids as some of them are the corticostEroids may ~revent the expected ...

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ژورنال

عنوان ژورنال: Nature

سال: 1955

ISSN: 0028-0836,1476-4687

DOI: 10.1038/175120a0